The RiverWATCH prototype program, developed for the Department of Homeland Security / FEMA and the Guntersville, AL Police Department, represents a significant advancement in water-based critical infrastructure protection. This comprehensive system is designed to provide rapid CBRNE (Chemical, Biological, Radiological, Nuclear, and Explosive) detection and response capabilities for port and river environments, focusing on the Port of Guntersville and the Tennessee River in North Alabama. At the heart of RiverWATCH is a high-speed vessel equipped with an integrated CRNE sensor package, enabling swift deployment and real-time monitoring of potential threats. This mobile platform is complemented by an array of tightly integrated sensors deployed across the port and surrounding land areas, creating a multi-layered detection network. The system's capabilities extend beyond mere detection, incorporating artificial intelligence for threat classification and dynamic plume modeling based on agent identification and current weather conditions. RiverWATCH leverages cutting-edge technology to ensure comprehensive situational awareness and responsive command and control. It utilizes a cloud-based, integrated mobile IT and CBRNE C4ISR (Command, Control, Communications, Computers, Intelligence, Surveillance, and Reconnaissance) solution. This is supported by a high-bandwidth, self-healing mesh network that enables robust communications between all system components, including mobile and fixed Command & Control (C2) stations and hand-held devices for on-the-go monitoring. The system's flexibility and adaptability are key features. Its plug-and-play architecture allows for easy integration of any IP-based sensor. A service-oriented architecture (SOA) approach in software design helps avoid single-point failures and ensures optimal performance. Communication is facilitated through multiple platforms, including 3G/4G, WiFi, WiMAX, and Satcom, providing redundancy and reliability in data transmission. RiverWATCH's real-time capabilities are particularly noteworthy. The system provides GIS- based CBRNE sensor information, allowing for precise localization of threats. Combined with integrated weather information for accurate plume modeling, this enables responders to make informed decisions quickly and effectively. Designed for Technology Readiness Level 9 (TRL-9), RiverWATCH is a fully operational system that supports 24/7 rapid deployment and sustainment operations. Its comprehensive coverage of water, port, and land-based threats, coupled with artificial intelligence-driven threat classification, positions it as a powerful tool for protecting critical infrastructure. Perhaps most importantly, RiverWATCH is designed with expandability in mind. While initially focused on the Port of Guntersville, the system's architecture allows for expansion to cover other water-based critical infrastructure along the Tennessee River, potentially serving as a model for similar deployments nationwide. The RiverWATCH prototype program represents a holistic approach to water-based security, combining advanced sensor technologies, robust networking, artificial intelligence, and flexible deployment options. RiverWATCH is a crucial advancement in protecting our nation's critical water-based infrastructure against CBRNE threats by providing real-time situational awareness and enhancing response capabilities.